Instruments
High Pressure Freezing
Leica EM ICE: rapid cryo-immobilization of aqueous samples while preserving their ultrastructure. Freezing at 5 times atmospheric pressure in liquid nitrogen within 1 second.
Software
Tomogram reconstructions
Tomographic reconstructions of cellular structures in Arabidopsis root cells.
(A) Tomographic slice and (B) tomographic reconstruction of an multivesicular endosome (MVE) with discrete vesicle networks (C) Free vesicles, single buds, and concatenated vesicle networks from the MVE shown in (A) and (B). Weiner et al (2024) PNAS
Comprehensive 3D reconstruction of synaptic ultrastructure at the Drosophila neuromuscular junction.
Comprehensive three-dimensional reconstruction of synaptic ultrastructure at the Drosophila NMJ. (A) A representative electron micrograph of a Drosophila NMJ synapse from a central position during a 120° tilt series of a 250-nm section. (B) A representative virtual slice of the tomogram generated from the tilt series in A. (C) 3D model of segmented features of the Drosophila NMJ synapse in A and B. Zhan et al (2016) J. Neurogenet