Podophrya collini
Coyle Lab
Arabidopsis root cells
Otegui lab
Nodavirus genome replication assembly
Ahlquist lab
Welcome to the 3D Cell EM Core Facility where we specialize in Electron Tomography of Cellular Structures Imaged at Room Temperature
Before working with the core
The core houses a Talos F200C microscope equipped with a high tilt holder which enable us to do electron tomography. This is a specialized imaging technique where images of a sample are collected at different tilt angles and reconstructed in a 3D volume of the sample. For sample preparation the core also has a Leica EM ICE high pressure freezer and a Leica UC7 ultramicrotome.
While working with the core
Electron microscopy is a valuable analytical tool but also highly specialized. We believe training and education is a vital part of supporting research and therefor provide training for users who are interested and assist with project planning where needed. Anyone interested in biological electron microscopy is welcome for a visit.
After working with the core
Please acknowledge the work done with the core using the statement below:
Some of this work was performed in the 3D Cell Electron Microscopy Core Facility in the Center for Quantitative Cell Imaging at the University of Wisconsin-Madison
Acknowledgement for the Leica EM ICE high pressure freezer:
The Leica EM ICE high pressure freezer was acquired with a National Institute of Health (1S10OD026769-01) grant.